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KMID : 0604520090350020135
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Journal of the Society of Cosmetic Scientists of Korea
2009 Volume.35 No. 2 p.135 ~ p.141
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Enzymatic Hydrolysis of Silk Sericin and Its Anti-oxidative Effect
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Kim Moo-Kon
Lee Jeong-Yong
Oh Han-Jin
Lee Ji-Young
Lee Ki-Hoon
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Abstract
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In this study, we hydrolyzed hot-water extracted sericin with single or two enzymes and investigated anti-oxidative effect on DPPH free radical and inhibitory effect on tyrosinase activity of the sericin hydrolysates. Alcalase, flavourzyme, and protamex were effective in hydrolyzing sericin. Sericin was degraded into the range of 20 ~ 30 kDa. The sericin hydrolysate was shown to have stronger antioxidant properties than the original sericin. In the case of flavourzyme and protamex combination, the scavenging effect of sericin hydrolysate on DPPH radical was increased up to about 85 %. However, the inhibitory effect on tyrosinase activity of enzymatic hydrolysates was lower than that of the original sericin. After fractionation of sericin hydrolysates, we found that F2 and P3 fraction has higher inhibitory effect on tyrosinase activity compared to other fractions.
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KEYWORD
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silk sericin, antioxidative effect, tyrosinase activity, enzymatic hydrolysis, flavourzyme
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